Academic Journal
Simultaneous Quantification of Antioxidants Paraxanthine and Caffeine in Human Saliva by Electrochemical Sensing for CYP1A2 Phenotyping.
Title: | Simultaneous Quantification of Antioxidants Paraxanthine and Caffeine in Human Saliva by Electrochemical Sensing for CYP1A2 Phenotyping. |
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Authors: | Anastasiadi, Rozalia-Maria, Berti, Federico, Colomban, Silvia, Tavagnacco, Claudio, Navarini, Luciano, Resmini, Marina |
Source: | Antioxidants; Jan2021, Vol. 10 Issue 1, p10-10, 1p |
Abstract: | The enzyme CYP1A2 is responsible for the metabolism of numerous antioxidants in the body, including caffeine, which is transformed into paraxanthine, its main primary metabolite. Both molecules are known for their antioxidant and pro-oxidant characteristics, and the paraxanthine-to-caffeine molar ratio is a widely accepted metric for CYP1A2 phenotyping, to optimize dose–response effects in individual patients. We developed a simple, cheap and fast electrochemical based method for the simultaneous quantification of paraxanthine and caffeine in human saliva, by differential pulse voltammetry, using an anodically pretreated glassy carbon electrode. Cyclic voltammetry experiments revealed for the first time that the oxidation of paraxanthine is diffusion controlled with an irreversible peak at ca. +1.24 V (vs. Ag/AgCl) in a 0.1 M H |
Subject Terms: | CARBON electrodes, SALIVA, CAFFEINE, CHARGE exchange, CYCLIC voltammetry |
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ISSN: | 20763921 |
DOI: | 10.3390/antiox10010010 |
Database: | Complementary Index |
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