Academic Journal
Biosynthesis of Furfurylamines in Batch and Continuous Flow by Immobilized Amine Transaminases.
| Title: | Biosynthesis of Furfurylamines in Batch and Continuous Flow by Immobilized Amine Transaminases. |
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| Authors: | Heinks, Tobias, Merz, Luisa M., Liedtke, Jan, Höhne, Matthias, van Langen, Luuk M., Bornscheuer, Uwe T., Fischer von Mollard, Gabriele, Berglund, Per |
| Source: | Catalysts (2073-4344); May2023, Vol. 13 Issue 5, p875, 15p |
| Abstract: | Building blocks with amine functionality are crucial in the chemical industry. Biocatalytic syntheses and chemicals derived from renewable resources are increasingly desired to achieve sustainable production of these amines. As a result, renewable materials such as furfurals, especially furfurylamines like 5-(hydroxymethyl)furfurylamine (HMFA) and 2,5-di(aminomethyl)furan (DAF), are gaining increasing attention. In this study, we identified four different amine transaminases (ATAs) that catalyze the reductive amination of 5-(hydroxymethyl)furfural (HMF) and 2,5-diformylfuran (DFF). We successfully immobilized these ATAs on glutaraldehyde-functionalized amine beads using multiple binding and on amine beads by site-selective binding of the unique Cα-formylglycine within an aldehyde tag. All immobilized ATAs were efficiently reused in five repetitive cycles of reductive amination of HMF with alanine as co-substrate, while the ATA from Silicibacter pomeroyi (ATA-Spo) also exhibited high stability for reuse when isopropylamine was used as an amine donor. Additionally, immobilized ATA-Spo yielded high conversion in the batch syntheses of HMFA and DAF using alanine (87% and 87%, respectively) or isopropylamine (99% and 98%, respectively) as amine donors. We further demonstrated that ATA-Spo was effective for the reductive amination of HMF with alanine or isopropylamine in continuous-flow catalysis with high conversion up to 12 days (48% and 41%, respectively). [ABSTRACT FROM AUTHOR] |
| Subject Terms: | AMINATION, AMINOTRANSFERASES, SUSTAINABILITY, AMINES, ISOPROPYLAMINE, BIOSYNTHESIS |
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| ISSN: | 20734344 |
| DOI: | 10.3390/catal13050875 |
| Database: | Complementary Index |
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