Test No. 237: Honey Bee (Apis Mellifera) Larval Toxicity Test, Single Exposure / Organisation for Economic Co-operation and Development.
This Test Guideline (TG) describes a honey bee brood acute toxicity test under laboratory conditions. The method aims at the determination of the lethal dose (72-h LD50) following single exposure of larvae to a chemical. On day 1 (D1) of the study, first instar synchronised larvae are taken from the...
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| Format: | Ebook |
| Language: | English |
| Published: |
Paris :
OECD Publishing,
2013.
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| Series: | OECD Guidelines for the Testing of Chemicals, Section 2,
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| Subjects: | |
| Online Access: | OECD |
| Summary: | This Test Guideline (TG) describes a honey bee brood acute toxicity test under laboratory conditions. The method aims at the determination of the lethal dose (72-h LD50) following single exposure of larvae to a chemical. On day 1 (D1) of the study, first instar synchronised larvae are taken from the comb of three colonies (thirty-six larvae in total per group) and individually placed into 48 well-plates where they are fed a standardized amount of artificial diet. On day 4 (D4) of the test, a single dose of the test chemical is administered to the larvae with the diet. Each group of thirty-six larvae is administered a given dose in a range of five increasing doses. Mortalities are recorded on D5, D6, and D7 of the test. The 72-hr LD50 is calculated for larvae (cumulative mortality at D7). The study report also includes a number of important elements, in particular regarding test conditions (e.g., temperature and humidity). |
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| Physical Description: | 1 online resource (10 pages). |
| Format: | Mode of access: World Wide Web. |
| ISBN: | 9264203753 9789264203754 |
| ISSN: | 2074-5761 |
| DOI: | 10.1787/9789264203723-en |