3D electro-rotation of single cells / Liang Huang, Wenhui Wang.
"Dielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost.However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electr...
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Main Authors: | , |
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Format: | Ebook |
Language: | English |
Published: |
Cham, Switzerland :
Springer,
[2020]
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Series: | Synthesis digital library of engineering and computer science.
Synthesis lectures on biomedical engineering ; #58. |
Subjects: | |
Online Access: | Springer Nature Synthesis Collection of Technology (R0) eBook Collection 2020 |
Summary: | "Dielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost.However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells.Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-rotation chip of "Armillary Sphere" was designed. The chip uses four thick electrodes and a bottom planar electrode to form an electric field chamber, which can control 3D rotation of single cells under different electric signal configurations. Electrical property measurement and 3D image reconstruction of single cells are achieved based on single-cell 3D rotation. This work overcomes the limitations of 2D planar electrodes and effectively solves the problem of unstable spatial position of single-cell samples, and provides a new platform for single-cell analysis.Based on multi-electrode structure of thick-electrode DEP, a microfluidic chip with optoelectronic integration was presented. A dual-fiber optical stretcher embedded in thick electrodes can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretching and rotation manipulation gives the chip the ability to simultaneously measure mechanical and electrical properties of single cells, providing a versatile platform for single-cell analysis, further extending the application of thick-electrode DEP in biological manipulation and analysis."--Publisher's website. |
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Item Description: | Part of: Synthesis digital library of engineering and computer science. |
Physical Description: | 1 online resource (xvii, 101 pages) : illustrations (some colour). |
Bibliography: | Includes bibliographical references. |
ISBN: | 1681736918 1681736926 1681736934 3031000455 3031016661 9781681736914 9781681736921 9781681736938 9783031000454 9783031016660 |
ISSN: | 1930-0336 ; |